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Part:BBa_K1559010

Designed by: Xiuqi (Rex) Xia   Group: iGEM14_Toronto   (2014-10-13)

RFP-Targeting protospacer for CRISPR/Cas9 system

This is a protospacer and PAM sequence that exists within the RFP coding sequence of Part:BBa_J04450. It allows Cas9 to cleave the DNA of the RFP plasmid at this site, causing the cell to lose the plasmid.

Usage and Biology

The protospacer and PAM forms the target site for the Cas9 nuclease. To make Cas9 target this site, the protospacer (not including the PAM) must be inserted into a crRNA array. (see Part:BBa_K1559002)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None